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CRISPR-FOCUS
A tool developed for high-throughput, rational sgRNA design in CRISPR screen experiment.
Select organism
Homo sapiens
Mus musculus
Options
Also design control sgRNAs
Avoid cancer recurrent mutation sites
Add promoter/scaffold sequences to the spacer
Paste your gene list here
(maximum 1000)
Number of sgRNAs for each gene
(maximum 30)
10
1
2
3
4
5
6
7
8
9
11
12
13
14
15
20
25
30
Spacer length
19nt
20nt
Submit